电离辐射通过转化内皮细胞-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨远红外线是否可通过转化生长因子-β（TGF-β）-依赖性的视网膜-间质转换 (EMT)来促进癌肝细胞的肆虐迁移。使用总量2Gy(60)Coγ线紫外线照射是从人类肾脏的6种癌肝细胞，记录与EMT引人注意的变化，这包括分别利用显微镜新科技，酶质印迹步骤，免疫荧光新科技，划痕试验和Transwell小室试验来注意到并检测肝细胞小组织形态，EMT标记，肆虐迁移并能等。使用酶排免疫吸附法检测这些癌肝细胞中的TGF-β酶技术水平，利用引人注意减缓剂SB431542来评估TGF-β信号通路在远红外线EMT中的的作用。经过总量为2Gy紫外线照射的癌肝细胞中的存在间叶肝细胞的表达，与假紫外线照射小组相比其视网膜标记减少，间叶肝细胞标记减少，同时其肆虐转移并能减弱，TGF-β酶技术水平也提高。进一步发现由A549远红外线游离的EMT可通过对TGF-β信号减缓牵涉到逆转。这些结果表明TGF-β依赖性的EMT在远红外线游离减弱癌肝细胞肆虐转移并能中的起着关键作用。

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